Melanotan II (MT-II) is a synthetic cyclic analogue of alpha-melanocyte-stimulating hormone supplied as a pre-filled research pen. Because the cyclic lactam structure and D-phenylalanine substitution distinguish it from linear peptides, purity verification confirms both the correct product peak and the absence of significant synthesis-related impurities. The Certificate of Analysis on this page documents Reverse-Phase HPLC purity of the Melanotan II cartridge on batch MT20034, alongside concentration, pH, and sterility results from the in-house release panel.
In-House Testing
BodyPharm laboratory
- Report
- #251210
- Batch
- MT20034
- Purity (HPLC)
- 99.37%
- Concentration
- 7.4 mg/ml
Testing Methodology
HPLC Purity of Melanotan II
BodyPharm in-house report #251210 documents Reverse-Phase HPLC analysis of batch MT20034 at 99.37% purity, with the Melanotan II peak resolving at a retention time of 16.42 minutes. The cyclic heptapeptide is well retained on a standard C18 column and resolves cleanly from its related substances. Related-substance peaks total 0.63% of relative area, with no individual impurity exceeding 0.31%, comfortably inside the not-more-than 2.0% individual and 5.0% total specification. Per-batch HPLC testing is repeated for every production run with a unique report number for traceability.
Concentration and Assay
The cartridge is filled to a target of 20mg Melanotan II in 3ml. Quantitative assay against a reference standard returned a concentration of 7.4 mg/ml, equating to a total API content of 22.2mg per 3ml cartridge - at or above the labelled content, which lets the multi-use pen deliver its full rated number of 0.1mg increments. The assay result is reported against the in-house acceptance criterion of not less than 6.3 mg/ml.
Formulation, Sterility and Storage
The finished solution is a colorless to light yellow liquid buffered to pH 7.0, with phenol at 5.0 mg/mL as the antimicrobial preservative. The batch conforms to sterility requirements and returned bacterial endotoxins below 20 EU/mg. Melanotan II is light-sensitive; storage at 2-8C in the original sealed pen, protected from light and freezing, preserves the peptide through its documented shelf life. The HPLC test reflects starting material at fill.
What This Result Means for Researchers
Melanotan II research applications centre on the melanocortin receptor family, where the peptide acts as a non-selective agonist across MC1R, MC3R, MC4R, and MC5R. Its best-characterised use is as a tool compound at MC1R in melanogenesis and pigmentation research, where receptor activation drives tyrosinase transcription and downstream melanin biosynthesis in cell and tissue models. The cyclic structure confers markedly greater potency and metabolic stability than native alpha-MSH, which is why Melanotan II is a standard reference agonist in melanocortin structure-activity research. The pre-filled pen provides a stable, refrigerated, sterile-filled formulation with precise 0.1mg volume increments for research protocols.
Frequently Asked Questions
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Melanotan II 20 Pen
Melanotan II 20mg pre-filled research pen, cyclic alpha-MSH analogue with HPLC-verified purity at 99.37%.